Topic 6 – Establishment of preclinical models in cancer : patient-derived xenografts (PDX) and innovative 3D organoid

Presentation

Patient-Derived tumor Xenografts (PDX) models conserved the heterogeneity of the tumors, and they are very useful for the identification of predictive biomarkers for therapeutic response and resistance. However, the take rate is far from being 100% for urological cancers, and it takes 18 months to develop them. It is thus difficult to come back to the patient to propose an adapted therapy. To circumvent such problem, our scientific project, initiated in the middle of 2019, is to develop more appropriate models, but complementary to PDX models. In this context, our goal is to develop 3D organoids for various cancer types including but not restricted to kidney cancer (KCa) and bladder cancer (BCa), from cells and tumors, considering the heterogeneity of the tumors by developing 3D structures from different parts of the tumor, containing all subcellular populations (tumor, stromal, immune cells) and in which we will include a vascularization through a microfluidic approach for drug testing. We have ideas for innovative tools. Our goal at term is to put in place an innovative platform combining both model types for translational and preclinical research, and personalized medicine.

p>Our project is thus addressing the urgent need to combine tumor model and vascularisation for more efficient and selective drug-screening model in cancer drug development. We will provide a ready-made prototype of new generation drug screening platform answering an important healthcare issue. 85% of new drugs are withdrawn at early stages of clinical trials due to inefficacy. Attrition rates in the development of especially cancer drugs are very high, often due to the lack of screening tools at the preclinical stage predictive enough to select potentially successful drug candidates. Thus, the added value is in the vascularized matrices, near what is observed in vivo, and allowing to analysis the therapeutic response to drugs reaching the subcellular populations of tumor cells, as it is the case when drugs are injected in patient, regardless of the route of application (iv, per os…).

We are therefore confident to provide a solution to today’s clear medical need of improved pre-clinical cancer drug candidate selection by providing a sophisticated, validated screening platform mimicking the tumor microenvironment to a high degree.

Scientific expertise

  • Cancerology
  • Onco-urology
  • Preclinical ‘study and research’ models (including CDX ‘Cell-Derived Xenograft’, PDX ‘Patient-Derived tumor Xenograft’; 3D organoids and tumoroids)
  • Development
  • Nephrology
  • Cardiovascular system
  • Cell and Molecular Biology
     

From fundamental, translational, to preclinical stages, including the search of new therapeutic options, the development of new preclinical tools, and the identification of diagnostic, prognostic, predictive and resistance biomarkers.

  • Scientific watch
  • ‘omic’ approaches
  • Transversality with other scientific domains such as chemistry, imagery, physics
  • Expertise in the evaluation of scientific programs, of recruiting folders and in the formation to research
     

Technical expertise

  • Development of PDX models in immunodefiscient mice for all cancer types (kidney, bladder, prostate, brain, lung…).
  • Development of 2D and 3D models of small (hanging drops technique in low attachment plates) and large scale (with the special technology from Cyphuse that allows to form 3D structures > 3 mm in diameter) from normal and tumoral tissues, derived from cell lines and tumors of patients (kidney, bladder, prostate, lung, glioblastome and virtually from all types of tumors) with a program that will allow to vascularize them and to add lymphoïdes matrices.
  • Proliferation and viability testings
  • Toxicity testing
  • Measurement of molecules efficacity… Therapeutic molecules or others
  • Development of 2D and 3D models – Also possible in coculture, 2 or more cell types
  • Tissue regeneration using various approaches
  • Study of the mechanism of action of therapeutic molecules, old or new or in repositioning
     

    • We have in our lab of the necessary light or heavy materials and facilities to ensure all these technologies, including, but not restricted to : Quantitative and non quantitative RT-PCR apparatus, Western blot apparatus, Electrospinnings, Isolators, PSM II, CYPHUSE Instrument S-PIKE, Cryostats, Microtomes, Miscrocopes (Fluorescent…), Rheometer… And also L2 laboratory.

    Let’s meet up !

    Always on the lookout for new public or private partners to support and help you in your research projects or to jointly develop and characterize new innovative systems and new fields of application for our Topic.
    Also please keep in mind that our Topic is also part of a state-of-the-art technology paltform that is currently setting-up in our laboratory ; contracts perform through Satt Conectus https://www.conectus.fr/ and on the way to be part of the Cortecs network of Strasbourg University https://cortecs.unistra.fr/.

    Team members of Topic 6

     

    Name
    		 Position
    		 Phone
    		 e-mail

    Thierry MASSFELDER
    INSERM Research Director
    +33 (0)3 68 85 34 56
    massfeld@unistra.fr

    Sabine BOPP-KUCHLER
    INSERM Researcher
    +33 (0)3 68 85 34 56
    kuchler@unistra.fr

    Ysia IDOUX-GILLET
    Research Engineer
    +33 (0)3 68 85 34 56
    yidouxgillet@unistra.fr

    Véronique LINDNER
    Associate professor, pathologist
    +33 (0)3 88 12 70 60
    veronique.lindner@chru-strasbourg.fr

    Claire MASSON
    Master 2 Student
    +33 (0)3 68 85 34 56
    claire.masson@etu.unistra.fr

    Laura KURZ-BERTAUX
    Master 1 Student
    +33 (0)3 68 85 34 56
    claura.kurz-bertaux@etu.unistra.fr

    Publications of Topic 6

    2021

    • Errera C, Gomart G, Lindner V, Bourcier T. Intra-ocular lens extrusion in a patient with corneal graft melting. Am J Ophthalmol Case Rep. 2021 Sep 15;24:101204. doi: 10.1016/j.ajoc.2021.101204. PMID: 34585022; PMCID: PMC8455978.

    • Felli E, Al-Taher M, Collins T, Nkusi R, Felli E, Baiocchini A, Lindner V, Vincent C, Barberio M, Geny B, Ettorre GM, Hostettler A, Mutter D, Gioux S, Schuster C, Marescaux J, Gracia-Sancho J, Diana M. Automatic Liver Viability Scoring with Deep Learning and Hyperspectral Imaging. Diagnostics (Basel). 2021 Aug 24;11(9):1527. doi: 10.3390/diagnostics11091527. PMID: 34573869; PMCID: PMC8472457.

    • Gerber V, Ruch Y, Chamaraux-Tran TN, Oulehri W, Schneider F, Lindner V, Greigert V, Denis J, Brunet J, Danion F. Detection of Pneumocystis jirovecii in Patients with Severe COVID-19: Diagnostic and Therapeutic Challenges. J Fungi (Basel). 2021 Jul 22;7(8):585. doi: 10.3390/jof7080585. PMID: 34436124; PMCID: PMC8397120.

    • Jühling F, Saviano A, Ponsolles C, Heydmann L, Crouchet E, Durand SC, El Saghire H, Felli E, Lindner V, Pessaux P, Pochet N, Schuster C, Verrier ER, Baumert TF. Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma. Life Sci Alliance. 2021 Jul 21;4(9):e202101036. doi: 10.26508/lsa.202101036. PMID: 34290079; PMCID: PMC8321681.

    • Lindner V, Waydelich A, Chen CC, Jones C, Stratton SP. Performance comparison of anti-p504s (SP116) Rabbit Monoclonal Primary Antibody vs. Monoclonal Rabbit Anti-Human AMACR clone 13H4 when duplexed with VENTANA Basal Cell Cocktail (34βE12+p63) as a diagnostic aid for prostatic adenocarcinoma using immunohistochemistry. Virchows Arch. 2021 Aug;479(2):337-343. doi: 10.1007/s00428-021-03088-4. Epub 2021 Apr 3. PMID: 33811532.

    • Marchegiani F, Noll E, Riva P, Kong SH, Saccomandi P, Vita G, Lindner V, Namer IJ, Marescaux J, Diemunsch P, Diana M. Effects of Warmed and Humidified CO2 Surgical Site Insufflation in a Novel Experimental Model of Magnetic Compression Colonic Anastomosis. Surg Innov. 2021 Feb;28(1):7-17. doi: 10.1177/1553350620967225. Epub 2020 Oct 23. PMID: 33095686.

    • Bornert F, Clauss F, Hua G, Idoux-Gillet Y, Keller L, Fernandez De Grado G, Offner D, Smaida R, Wagner Q, Fioretti F, Kuchler-Bopp S, Schulz G, Wenzel W, Gentile L, Risser L, Müller B, Huck O, Benkirane-Jessel N. Mechanistic Illustration: How Newly-Formed Blood Vessels Stopped by the Mineral Blocks of Bone Substitutes Can Be Avoided by Using Innovative Combined Therapeutics. Biomedicines. 2021 Aug 3;9(8):952. doi: 10.3390/biomedicines9080952. PMID: 34440156; PMCID: PMC8394928.

    • Stutz C, Clauss F, Huck O, Schulz G, Benkirane-Jessel N, Bornert F, Kuchler-Bopp S, Strub M. Eruption of Bioengineered Teeth: A New Approach Based on a Polycaprolactone Biomembrane. Nanomaterials (Basel). 2021 May 17;11(5):1315. doi: 10.3390/nano11051315. PMID: 34067681; PMCID: PMC8156264.

    2020

    • Hassan S, Peluso J, Chalhoub S, Idoux Gillet Y, Benkirane-Jessel N, Rochel N, Fuhrmann G, Ubeaud-Sequier G. Quercetin potentializes the respective cytotoxic activity of gemcitabine or doxorubicin on 3D culture of AsPC-1 or HepG2 cells, through the inhibition of HIF-1α and MDR1. PLoS One. 2020 Oct 14;15(10):e0240676. doi: 10.1371/journal.pone.0240676. PMID: 33052979; PMCID: PMC7556446.

    • Harmouch E, Seitlinger J, Chaddad H, Ubeaud-Sequier G, Barths J, Saidu S, Désaubry L, Grandemange S, Massfelder T, Fuhrmann G, Fioretti F, Dontenwill M, Benkirane-Jessel N, Idoux-Gillet Y. Flavagline synthetic derivative induces senescence in glioblastoma cancer cells without being toxic to healthy astrocytes. Sci Rep. 2020 Aug 13;10(1):13750. doi: 10.1038/s41598-020-70820-6. PMID: 32792639; PMCID: PMC7426813.

    • Stutz C, Batool F, Petit C, Strub M, Kuchler-Bopp S, Benkirane-Jessel N, Huck O. Influence of parathyroid hormone on periodontal healing in animal models: A systematic review. Arch Oral Biol. 2020 Dec;120:104932. doi: 10.1016/j.archoralbio.2020.104932. Epub 2020 Oct 6. PMID: 33113458.

    • Stutz C, Strub M, Clauss F, Huck O, Schulz G, Gegout H, Benkirane-Jessel N, Bornert F, Kuchler-Bopp S. A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration. Nanomaterials (Basel). 2020 Sep 8;10(9):1774. doi: 10.3390/nano10091774. PMID: 32911737; PMCID: PMC7558050.

    • Smaida R, Pijnenburg L, Irusta S, Himawan E, Mendoza G, Harmouch E, Idoux-Gillet Y, Kuchler-Bopp S, Benkirane-Jessel N, Hua G. Potential Implantable Nanofibrous Biomaterials Combined with Stem Cells for Subchondral Bone Regeneration. Materials (Basel). 2020 Jul 10;13(14):3087. doi: 10.3390/ma13143087. PMID: 32664278; PMCID: PMC7412392.

    • Favreau H, Pijnenburg L, Seitlinger J, Fioretti F, Keller L, Scipioni D, Adriaensen H, Kuchler-Bopp S, Ehlinger M, Mainard D, Rosset P, Hua G, Gentile L, Benkirane-Jessel N. Osteochondral repair combining therapeutics implant with mesenchymal stem cells spheroids. Nanomedicine. 2020 Oct;29:102253. doi: 10.1016/j.nano.2020.102253. Epub 2020 Jul 1. PMID: 32619705.

    • Kuchler-Bopp S, Mariotte A, Strub M, Po C, De Cauwer A, Schulz G, Van Bellinghen X, Fioretti F, Clauss F, Georgel P, Benkirane-Jessel N, Bornert F. Temporomandibular joint damage in K/BxN arthritic mice. Int J Oral Sci. 2020 Feb 6;12(1):5. doi: 10.1038/s41368-019-0072-z. PMID: 32024813; PMCID: PMC7002582.

    • Marchegiani F, Noll E, Riva P, Kong SH, Saccomandi P, Vita G, Lindner V, Namer IJ, Marescaux J, Diemunsch P, Diana M. Effects of Warmed and Humidified CO2 Surgical Site Insufflation in a Novel Experimental Model of Magnetic Compression Colonic Anastomosis. Surg Innov. 2021 Feb;28(1):7-17. doi: 10.1177/1553350620967225. Epub 2020 Oct 23. PMID: 33095686.

    • Leroy-Freschini B, Lindner V, Boisramé T, Demarchi M. 18F-FDG PET/CT Imaging of Peritoneal Fibrosis Mimicking Persistent Metastatic Ovarian Carcinoma. Nucl Med Mol Imaging. 2020 Oct;54(5):249-251. doi: 10.1007/s13139-020-00656-5. Epub 2020 Jul 25. PMID: 33088354; PMCID: PMC7560674.

    • Barberio M, Felli E, Pop R, Pizzicannella M, Geny B, Lindner V, Baiocchini A, Jansen-Winkeln B, Moulla Y, Agnus V, Marescaux J, Gockel I, Diana M. A Novel Technique to Improve Anastomotic Perfusion Prior to Esophageal Surgery: Hybrid Ischemic Preconditioning of the Stomach. Preclinical Efficacy Proof in a Porcine Survival Model. Cancers (Basel). 2020 Oct 14;12(10):2977. doi: 10.3390/cancers12102977. PMID: 33066529; PMCID: PMC7602144.

    • Felli E, Al-Taher M, Collins T, Baiocchini A, Felli E, Barberio M, Ettorre GM, Mutter D, Lindner V, Hostettler A, Gioux S, Schuster C, Marescaux J, Diana M. Hyperspectral evaluation of hepatic oxygenation in a model of total vs. arterial liver ischaemia. Sci Rep. 2020 Sep 22;10(1):15441. doi: 10.1038/s41598-020-72915-6. PMID: 32963333; PMCID: PMC7509803.

    • Herrscher H, Boilève A, Lindner V, Barthélémy P, Hutt É, Pierard L, Kurtz JE, Rioux-Leclercq N, Lang H, Malouf GG. Les carcinomes du rein à translocation de la famille MiT : histoire naturelle, caractéristiques moléculaire et prise en charge multidisciplinaire [MiT family translocation renal cell carcinomas: Natural history, molecular features and multidisciplinary management]. Bull Cancer. 2020 Feb;107(2):272-280. French. doi: 10.1016/j.bulcan.2019.11.010. Epub 2020 Feb 7. PMID: 32044098.

    • Gosvig K, Jensen SS, Qvist N, Agnus V, Jensen TS, Lindner V, Marescaux J, Diana M, Ellebæk MB. Remote computer-assisted analysis of ICG fluorescence signal for evaluation of small intestinal anastomotic perfusion: a blinded, randomized, experimental trial. Surg Endosc. 2020 May;34(5):2095-2102. doi: 10.1007/s00464-019-06990-w. Epub 2019 Jul 22. PMID: 31332565.

    • Barberio M, Longo F, Fiorillo C, Seeliger B, Mascagni P, Agnus V, Lindner V, Geny B, Charles AL, Gockel I, Worreth M, Saadi A, Marescaux J, Diana M. HYPerspectral Enhanced Reality (HYPER): a physiology-based surgical guidance tool. Surg Endosc. 2020 Apr;34(4):1736-1744. doi: 10.1007/s00464-019-06959-9. Epub 2019 Jul 15. PMID: 31309313.

    • Gaullier M, Tricard T, Garnon J, Cazzato RL, Munier P, De Marini P, Werle P, Lindner V, Gangi A, Lang H. Cryothérapie totale de la prostate sous IRM par voie périnéale : résultats carcinologiques et facteurs prédictifs [Percutaneous MR-guided prostate cancer cryoablation: Predictive factors and oncologic outcomes]. Prog Urol. 2020 Jan;30(1):12-18. French. doi: 10.1016/j.purol.2019.10.006. Epub 2019 Dec 11. PMID: 31837926.

    • Lassalle M, Beraud C, Lang H, Lindner V, Allory Y, Potiron E, Massfelder T, Lluel P, Misseri Y. Mutational landscape and pharmacological profiling of a panel of prostate PDX models including hormone-naive, hormone-sensitive and castrate-resistant prostate cancer specimens. Cancer Res, 2020, Meeting abstract: 1668 Volume: 80 Issue: 16 DOI: 10.1158/1538-7445.AM2020-1668 Supplement.

    • Beraud C, Lang H, Lassalle M, Lindner V, Kamoun A, Soulie M, Guillon E, Krucker C, Game X, Rischmann P, De Reynies A, Allory Y, Radvanyi F, Lluel P, Massfelder T, Bernard-Pierrot I. Establishment of a panel of patient-derived tumor xenograft models recapitulating molecular heterogeneity and drug response of muscle-invasive bladder tumors. Clin Cancer Res, 2020, Meeting abstract: A24 Volume: 26 Issue: 15 Pages: 43-44 Supplement.

    2019

    • Keller L, Pijnenburg L, Idoux-Gillet Y, Bornert F, Benameur L, Tabrizian M, Auvray P, Rosset P, María Gonzalo-Daganzo R, Gómez Barrena E, Gentile L, Benkirane-Jessel N. Preclinical safety study of a combined therapeutic bone wound dressing for osteoarticular regeneration. Nat Commun. 2019 May 14;10(1):2156. doi: 10.1038/s41467-019-10165-5. PMID: 31089136; PMCID: PMC6517440.

    • Rochel N, Krucker C, Coutos-Thévenot L, Osz J, Zhang R, Guyon E, Zita W, Vanthong S, Hernandez OA, Bourguet M, Badawy KA, Dufour F, Peluso-Iltis C, Heckler-Beji S, Dejaegere A, Kamoun A, de Reyniès A, Neuzillet Y, Rebouissou S, Béraud C, Lang H, Massfelder T, Allory Y, Cianférani S, Stote RH, Radvanyi F, Bernard-Pierrot I. Recurrent activating mutations of PPARγ associated with luminal bladder tumors. Nat Commun. 2019 Jan 16;10(1):253. doi: 10.1038/s41467-018-08157-y. PMID: 30651555; PMCID: PMC6335423.

    • Hamaidi I, Coquard C, Danilin S, Dormoy V, Béraud C, Rothhut S, Barthelmebs M, Benkirane-Jessel N, Lindner V, Lang H, Massfelder T. The Lim1 oncogene as a new therapeutic target for metastatic human renal cell carcinoma. Oncogene. 2019 Jan;38(1):60-72. doi: 10.1038/s41388-018-0413-y. Epub 2018 Aug 3. PMID: 30076415.

    • Lang H, Beraud C, Lasslle M, Lindner V, Potiron E, Lluel P, Massfelder T. Characterization of hormone-sensitive and castrate-resistant phenotypes in prostate cancer patient derived PDX models generated from the same patient. Cancer Res, 2019, Meeting abstract: 92 Volume: 79 Issue: 13 DOI: 10.1158/1538-7445.SABCS18-92 Supplement.

    • Lang H, Beraud C, Lassalle M, Lindner V, Saulie M, Game X, Rischmann P, Allory Y, Radvanyi F, Bernard-Pierrot I, Lluel P, Massfelder T. High specific characterization of patient-derived tumor xenograft models for accelerating drug development in muscle-invasive bladder cancers. Cancer Res, 2019, Meeting abstract: 1930 Volume: 79 Issue: 13 DOI: 10.1158/1538-7445.AM2019-1930 Supplement.

    • Zein N, Harmouch E, Lutz JC, Fernandez De Grado G, Kuchler-Bopp S, Clauss F, Offner D, Hua G, Benkirane-Jessel N, Fioretti F. Polymer-Based Instructive Scaffolds for Endodontic Regeneration. Materials (Basel). 2019 Jul 24;12(15):2347. doi: 10.3390/ma12152347. PMID: 31344822; PMCID: PMC6695966.

    • Debien V, Thouvenin J, Lindner V, Barthélémy P, Lang H, Flippot R, Malouf GG. Sarcomatoid Dedifferentiation in Renal Cell Carcinoma: From Novel Molecular Insights to New Clinical Opportunities. Cancers (Basel). 2019 Dec 31;12(1):99. doi: 10.3390/cancers12010099. PMID: 31906050; PMCID: PMC7016737.

    • Giménez ME, Davrieux CF, Serra E, Palermo M, Houghton EJ, Alonci G, Piantanida E, Garcia Vazquez A, Lindner V, Dallemagne B, Diana M, Marescaux J, De Cola L. Application of a novel material in the inguinal region using a totally percutaneous approach in an animal model: a new potential technique? Hernia. 2019 Dec;23(6):1175-1185. doi: 10.1007/s10029-019-01999-5. Epub 2019 Jul 16. PMID: 31312941.

    • Ignat M, Lindner V, Vix M, Marescaux J, Mutter D. Intraoperative Probe-Based Confocal Endomicroscopy to Histologically Differentiate Thyroid From Parathyroid Tissue Before Resection. Surg Innov. 2019 Apr;26(2):141-148. doi: 10.1177/1553350618814078. Epub 2018 Nov 23. PMID: 30466375.

    2018

    • Sy PM, Anton N, Idoux-Gillet Y, Dieng SM, Messaddeq N, Ennahar S, Diarra M, Vandamme TF. Pickering nano-emulsion as a nanocarrier for pH-triggered drug release. Int J Pharm. 2018 Oct 5;549(1-2):299-305. doi: 10.1016/j.ijpharm.2018.07.066. Epub 2018 Jul 31. PMID: 30075249.

    • Fernandez de Grado G, Keller L, Idoux-Gillet Y, Wagner Q, Musset AM, Benkirane-Jessel N, Bornert F, Offner D. Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management. J Tissue Eng. 2018 Jun 4;9:2041731418776819. doi: 10.1177/2041731418776819. PMID: 29899969; PMCID: PMC5990883.

    • Mahe M, Dufour F, Neyret-Kahn H, Moreno-Vega A, Beraud C, Shi M, Hamaidi I, Sanchez-Quiles V, Krucker C, Dorland-Galliot M, Chapeaublanc E, Nicolle R, Lang H, Pouponnot C, Massfelder T, Radvanyi F, Bernard-Pierrot I. An FGFR3/MYC positive feedback loop provides new opportunities for targeted therapies in bladder cancers. EMBO Mol Med. 2018 Apr;10(4):e8163. doi: 10.15252/emmm.201708163. PMID: 29463565; PMCID: PMC5887543.

    • Hochane M, Raison D, Coquard C, Béraud C, Danilin S, Bethry A, Massfelder T, Barthelmebs M. Parathyroid Hormone-Related Protein Contributes to Early Healing of Habu Snake Venom-Induced Glomerulonephritis in Mice. Am J Pathol. 2018 Apr;188(4):863-875. doi: 10.1016/j.ajpath.2017.12.012. Epub 2018 Jan 17. PMID: 29353060.

    • Neuberg P, Hamaidi I, Danilin S, Ripoll M, Lindner V, Nothisen M, Wagner A, Kichler A, Massfelder T*, Remy JS*. Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery. Nanoscale. 2018 Jan 25;10(4):1587-1590. doi: 10.1039/c7nr09202d. PMID: 29322141. * Corresponding authors.

    • Lang H, Beraud C, Lassalle M, Bernard-Pierrot I, Lindner V, Allory Y, Soulie M, Game X, Rischmann P, Potiron E, Radvanyi F, Lluel P, Massfelder T. A comprehensive patient-derived tumor xenograft (PDX) collection representing the heterogeneity of kidney, prostate and bladder cancers. Cancer Res, 2018, Meeting abstract: 1035 Volume: 78 Issue: 13 DOI: 10.1158/1538-7445.AM2018-1035 Supplement.

    • Hochane M, Raison D, Coquard C, Béraud C, Bethry A, Danilin S, Massfelder T, Barthelmebs M. Parathyroid hormone-related protein modulates inflammation in mouse mesangial cells and blunts apoptosis by enhancing COX-2 expression. Am J Physiol Cell Physiol. 2018 Feb 1;314(2):C242-C253. doi: 10.1152/ajpcell.00018.2017. Epub 2017 Nov 15. PMID: 29141920.

    • Bugueno IM, Batool F, Keller L, Kuchler-Bopp S, Benkirane-Jessel N, Huck O. Porphyromonas gingivalis bypasses epithelial barrier and modulates fibroblastic inflammatory response in an in vitro 3D spheroid model. Sci Rep. 2018 Oct 8;8(1):14914. doi: 10.1038/s41598-018-33267-4. PMID: 30297793; PMCID: PMC6175856.

    • Strub M, Keller L, Idoux-Gillet Y, Lesot H, Clauss F, Benkirane-Jessel N, Kuchler-Bopp S. Bone Marrow Stromal Cells Promote Innervation of Bioengineered Teeth. J Dent Res. 2018 Sep;97(10):1152-1159. doi: 10.1177/0022034518779077. Epub 2018 Jun 7. PMID: 29879365.

    • Batool F, Strub M, Petit C, Bugueno IM, Bornert F, Clauss F, Huck O, Kuchler-Bopp S, Benkirane-Jessel N. Periodontal Tissues, Maxillary Jaw Bone, and Tooth Regeneration Approaches: From Animal Models Analyses to Clinical Applications. Nanomaterials (Basel). 2018 May 16;8(5):337. doi: 10.3390/nano8050337. PMID: 29772691; PMCID: PMC5977351.

    • Strub M, Van Bellinghen X, Fioretti F, Bornert F, Benkirane-Jessel N, Idoux-Gillet Y, Kuchler-Bopp S, Clauss F. Maxillary Bone Regeneration Based on Nanoreservoirs Functionalized ε-Polycaprolactone Biomembranes in a Mouse Model of Jaw Bone Lesion. Biomed Res Int. 2018 Feb 26;2018:7380389. doi: 10.1155/2018/7380389. PMID: 29682553; PMCID: PMC5846386.

    • Kuchler-Bopp S, Bagnard D, Van-Der-Heyden M, Idoux-Gillet Y, Strub M, Gegout H, Lesot H, Benkirane-Jessel N, Keller L. Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth. J Tissue Eng Regen Med. 2018 Apr;12(4):e2151-e2161. doi: 10.1002/term.2648. Epub 2018 Mar 25. PMID: 29430872.

    • Van Bellinghen X, Idoux-Gillet Y, Pugliano M, Strub M, Bornert F, Clauss F, Schwinté P, Keller L, Benkirane-Jessel N, Kuchler-Bopp S, Lutz JC, Fioretti F. Temporomandibular Joint Regenerative Medicine. Int J Mol Sci. 2018 Feb 2;19(2):446. doi: 10.3390/ijms19020446. PMID: 29393880; PMCID: PMC5855668.

    • Silva AKA, Perretta S, Perrod G, Pidial L, Lindner V, Carn F, Lemieux S, Alloyeau D, Boucenna I, Menasché P, Dallemagne B, Gazeau F, Wilhelm C, Cellier C, Clément O, Rahmi G. Thermoresponsive Gel Embedded with Adipose Stem-Cell-Derived Extracellular Vesicles Promotes Esophageal Fistula Healing in a Thermo-Actuated Delivery Strategy. ACS Nano. 2018 Oct 23;12(10):9800-9814. doi: 10.1021/acsnano.8b00117. Epub 2018 Sep 25. PMID: 30231208.

    • Pop R, Mihoc D, Manisor M, Richter JS, Lindner V, Janssen-Langenstein R, Simu M, Wolff V, Beaujeux R. Mechanical thrombectomy for repeated cerebral tumor embolism from a thoracic sarcomatoid carcinoma. J Neurointerv Surg. 2018 Oct;10(10):e26. doi: 10.1136/neurintsurg-2017-013092.rep. Epub 2018 May 24. PMID: 29794158.

    • Nouhaud FX, Bernhard JC, Bigot P, Khene ZE, Audenet F, Lang H, Bergerat S, Fraisse G, Grenier N, Cornelis F, Nedelcu C, Béjar S, Fromont-Hankard G, Allory Y, Lindner V, Verkarre V, Daniel L, Yacoub M, Correas JM, Méjean A, Rioux-Leclercq N, Bensalah K. Contemporary assessment of the correlation between Bosniak classification and histological characteristics of surgically removed atypical renal cysts (UroCCR-12 study). World J Urol. 2018 Oct;36(10):1643-1649. doi: 10.1007/s00345-018-2307-6. Epub 2018 May 5. PMID: 29730837.

    • Korenbaum C, Pierard L, Thiéry A, Story F, Lindner V, Lang H, Kurtz JE, Barthélémy P. Treatments, Outcomes, and Validity of Prognostic Scores in Patients With Sarcomatoid Renal Cell Carcinoma: A 20-Year Single-Institution Experience. Clin Genitourin Cancer. 2018 Jun;16(3):e577-e586. doi: 10.1016/j.clgc.2017.12.005. Epub 2017 Dec 28. PMID: 29395949.

    • Diana M, Noll E, Legnèr A, Kong SH, Liu YY, Schiraldi L, Marchegiani F, Bano J, Geny B, Charles AL, Dallemagne B, Lindner V, Mutter D, Diemunsch P, Marescaux J. Impact of valve-less vs. standard insufflation on pneumoperitoneum volume, inflammation, and peritoneal physiology in a laparoscopic sigmoid resection experimental model. Surg Endosc. 2018 Jul;32(7):3215-3224. doi: 10.1007/s00464-018-6039-x. Epub 2018 Jan 12. PMID: 29330589.

    • Tricard T, Addeo P, Sauvinet G, Mouracadé P, Lindner V, Le Van Quyen P, Roy C, Lang H, Bachellier P. Maladie aux IgG-4 : piège pour les urologues ? [IgG4-related kidney disease: Urologist trap?]. Prog Urol. 2018 Mar;28(3):135-136. French. doi: 10.1016/j.purol.2017.11.001. Epub 2017 Dec 6. PMID: 29203156.

    2017

    • Acuna-Mendoza S, Martin S, Kuchler-Bopp S, Ribes S, Thalgott J, Chaussain C, Creuzet S, Lesot H, Lebrin F, Poliard A. A New Wnt1-CRE TomatoRosa Embryonic Stem Cell Line: A Tool for Studying Neural Crest Cell Integration Capacity. Stem Cells Dev. 2017 Dec 1;26(23):1682-1694. doi: 10.1089/scd.2017.0115. Epub 2017 Nov 3. PMID: 28922973.

    • Chaddad H, Kuchler-Bopp S, Fuhrmann G, Gegout H, Ubeaud-Sequier G, Schwinté P, Bornert F, Benkirane-Jessel N, Idoux-Gillet Y. Combining 2D angiogenesis and 3D osteosarcoma microtissues to improve vascularization. Exp Cell Res. 2017 Nov 15;360(2):138-145. doi: 10.1016/j.yexcr.2017.08.035. Epub 2017 Sep 1. PMID: 28867479.

    • Offner D, Wagner Q, Idoux-Gillet Y, Gegout H, Ferrandon A, Schwinté P, Musset AM, Benkirane-Jessel N, Keller L. Hybrid collagen sponge and stem cells as a new combined scaffold able to induce the re-organization of endothelial cells into clustered networks. Biomed Mater Eng. 2017;28(s1):S185-S192. doi: 10.3233/BME-171640. PMID: 28372294.

    • Keller L, Idoux-Gillet Y, Wagner Q, Eap S, Brasse D, Schwinté P, Arruebo M, Benkirane-Jessel N. Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids. Int J Nanomedicine. 2017 Jan 12;12:447-457. doi: 10.2147/IJN.S116749. PMID: 28138241; PMCID: PMC5238755.

    • Kuchler-Bopp S, Larrea A, Petry L, Idoux-Gillet Y, Sebastian V, Ferrandon A, Schwinté P, Arruebo M, Benkirane-Jessel N. Promoting bioengineered tooth innervation using nanostructured and hybrid scaffolds. Acta Biomater. 2017 Mar 1;50:493-501. doi: 10.1016/j.actbio.2017.01.001. Epub 2017 Jan 3. PMID: 28057509.

    • Tricard T, Tsoumakidou G, Lindner V, Garnon J, Albrand G, Cathelineau X, Gangi A, Lang H. Thérapies ablatives dans le cancer du rein : indications [Ablative therapy in kidney cancer: Indications]. Prog Urol. 2017 Nov;27(15):926-951. French. doi: 10.1016/j.purol.2017.07.245. Epub 2017 Aug 30. PMID: 28869173.

    • Pop R, Mihoc D, Manisor M, Richter JS, Lindner V, Janssen-Langenstein R, Simu M, Wolff V, Beaujeux R. Mechanical thrombectomy for repeated cerebral tumor embolism from a thoracic sarcomatoid carcinoma. BMJ Case Rep. 2017 Aug 11;2017:bcr2017013092. doi: 10.1136/bcr-2017-013092. PMID: 28801324; PMCID: PMC5753705.

    • Derquin F, Moreau S, Dargent F, Rioux Leclercq N; les membres du réseau Carare. Carcinome rénal tubulokystique avec composante peu différenciée [Tubulocystic renal cell carcinoma with poorly differentiated foci]. Ann Pathol. 2017 Aug;37(4):318-320. French. doi: 10.1016/j.annpat.2017.05.003. Epub 2017 Jul 18. PMID: 28732560.

    • Le Van Quyen P, Desprez P, Livolsi A, Lindner V, Fafi-Kremer S, Helms P, Antal MC. Peculiar Clinical Presentation of Coxsackievirus B4 Infection: Neonatal Restrictive Cardiomyopathy. AJP Rep. 2017 Apr;7(2):e124-e126. doi: 10.1055/s-0037-1601352. Epub 2017 Jun 29. PMID: 28670500; PMCID: PMC5491342.

    • Bergerat S, Barthelemy P, Mouracade P, Lang H, Saussine C, Lindner V, Jacqmin D. Primary CIC-DUX4 round cell sarcoma of the kidney: A treatment-refractory tumor with poor outcome. Pathol Res Pract. 2017 Feb;213(2):154-160. doi: 10.1016/j.prp.2016.11.015. Epub 2016 Nov 24. PMID: 27919577.

    • Akladios C, De Ruijter V, Perretta S, Aprahamian M, Ignat M, Lindner V, Averous G, Dallemagne B, Marescaux J. Feasibility and reliability of pancreatic cancer staging using a new confocal non-fluorescent microscopy probe: a double-blind study in rats. Surg Endosc. 2017 Feb;31(2):995-1003. doi: 10.1007/s00464-016-5062-z. Epub 2016 Jun 28. PMID: 27352785.

    • Diana M, Noll E, Charles AL, Diemunsch P, Geny B, Liu YY, Marchegiani F, Schiraldi L, Agnus V, Lindner V, Swanström L, Dallemagne B, Marescaux J. Precision real-time evaluation of bowel perfusion: accuracy of confocal endomicroscopy assessment of stoma in a controlled hemorrhagic shock model. Surg Endosc. 2017 Feb;31(2):680-691. doi: 10.1007/s00464-016-5022-7. Epub 2016 Jun 20. PMID: 27324327.